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CDK6 (Phospho Tyr24) antibody

Anti-CDK6 (Phospho Tyr24) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50353
Anti-CDK6 (Phospho Tyr24) antibody used in IHC (Paraffin sections) (IHC-P). GTX50353
Anti-CDK6 (Phospho Tyr24) antibody used in Western Blot (WB). GTX50353

Cat No. GTX50353

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 24 (G-A-Y(p)-G-K) derived from human CDK6.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase 6 , MCPH12 , PLSTIRE

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-CDK6 (Phospho Tyr24) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50353

GTX50353 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50353 CDK6 (Phospho Tyr24) antibody.

Anti-CDK6 (Phospho Tyr24) antibody used in IHC (Paraffin sections) (IHC-P). GTX50353

GTX50353 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX50353 CDK6 (Phospho Tyr24) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-CDK6 (Phospho Tyr24) antibody used in Western Blot (WB). GTX50353

GTX50353 WB Image

WB analysis of extracts from 293 cells untreated (lane 1) or treated with starvation (lane 2) using GTX50353 CDK6 (Phospho Tyr24) antibody.

REFERENCE

There are currently no references for CDK6 (Phospho Tyr24) antibody (GTX50353). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf