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CDK6 antibody [HL2078]

Recombinant antibody Orthogonal Overexpression
Anti-CDK6 antibody [HL2078] used in Western Blot (WB). GTX637986
Anti-CDK6 antibody [HL2078] used in Western Blot (WB). GTX637986
Anti-CDK6 antibody [HL2078] used in IHC (Paraffin sections) (IHC-P). GTX637986
Anti-CDK6 antibody [HL2078] used in IHC (Paraffin sections) (IHC-P). GTX637986
Anti-CDK6 antibody [HL2078] used in Western Blot (WB). GTX637986

Cat. No. GTX637986

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2078

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CDK6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human CDK6.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 6 , MCPH12 , PLSTIRE

Cellular Localization

Cytoplasm,Nucleus,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Background

The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-CDK6 antibody [HL2078] used in Western Blot (WB). GTX637986

GTX637986 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [HL2078] (GTX637986) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CDK6 antibody [HL2078] used in Western Blot (WB). GTX637986

GTX637986 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [HL2078] (GTX637986) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK6 antibody [HL2078] used in IHC (Paraffin sections) (IHC-P). GTX637986

GTX637986 IHC-P Image

CDK6 antibody [HL2078] detects CDK6 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
CDK6 stained by CDK6 antibody [HL2078] (GTX637986) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CDK6 antibody [HL2078] used in IHC (Paraffin sections) (IHC-P). GTX637986

GTX637986 IHC-P Image

CDK6 antibody [HL2078] detects CDK6 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
CDK6 stained by CDK6 antibody [HL2078] (GTX637986) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CDK6 antibody [HL2078] used in Western Blot (WB). GTX637986

GTX637986 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [HL2078] (GTX637986) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations