GeneTex
United States (US)

CDK6 antibody [N1C3]

GTX103992_WB_19011506_798.jpg
GTX103992_40135_20151227_IHC-P_19011506_952.jpg
GTX103992_40135_20141219_IHC_19011506_994.jpg
GTX103992_IFA_19011506_137.jpg

Cat No. GTX103992

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDK6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase 6,Mcph12,Plstire,Cdk6

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103992_WB_19011506_798.jpg

GTX103992 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX103992 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103992_40135_20151227_IHC-P_19011506_952.jpg

GTX103992 IHC-P Image

CDK6 antibody [N1C3] detects CDK6 protein at cytoplasm and nucleus in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
CDK6 antibody [N1C3] (GTX103992) diluted at 1:500.

GTX103992_40135_20141219_IHC_19011506_994.jpg

GTX103992 IHC-P Image

CDK6 antibody [N1C3] detects CDK6 protein at cytoplasm and nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
CDK6 antibody [N1C3] (GTX103992) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103992_IFA_19011506_137.jpg

GTX103992 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CDK6(GTX103992) antibody at 1:200 dilution.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169