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CDK6 antibody [N3C3]

Overexpression
Anti-CDK6 antibody [N3C3] used in Western Blot (WB). GTX113159
Anti-CDK6 antibody [N3C3] used in Western Blot (WB). GTX113159
Anti-CDK6 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113159
Anti-CDK6 antibody [N3C3] used in Western Blot (WB). GTX113159

Cat. No. GTX113159

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CDK6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

IMR32 , Jurkat , Raji , NCI-H929

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDK6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178748

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 6 , MCPH12 , PLSTIRE

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDK6 antibody [N3C3] used in Western Blot (WB). GTX113159

GTX113159 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N3C3] (GTX113159) diluted at 1:500.

Anti-CDK6 antibody [N3C3] used in Western Blot (WB). GTX113159

GTX113159 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N3C3] (GTX113159) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK6 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113159

GTX113159 ICC/IF Image

CDK6 antibody [N3C3] detects CDK6 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDK6 protein stained by CDK6 antibody [N3C3] (GTX113159) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-CDK6 antibody [N3C3] used in Western Blot (WB). GTX113159

GTX113159 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
10% SDS PAGE
GTX113159 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169