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CDK6 antibody [N3C3]

Anti-CDK6 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113159
Anti-CDK6 antibody [N3C3] used in Western Blot (WB). GTX113159
Anti-CDK6 antibody [N3C3] used in Western Blot (WB). GTX113159

Cat No. GTX113159

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

IMR32 , Jurkat , Raji , NCI-H929

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDK6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase 6,Mcph12,Plstire,Cdk6

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CDK6 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113159

GTX113159 ICC/IF Image

CDK6 antibody [N3C3] detects CDK6 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDK6 protein stained by CDK6 antibody [N3C3] (GTX113159) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-CDK6 antibody [N3C3] used in Western Blot (WB). GTX113159

GTX113159 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
10% SDS PAGE
GTX113159 diluted at 1:1000

Anti-CDK6 antibody [N3C3] used in Western Blot (WB). GTX113159

GTX113159 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N3C3] (GTX113159) diluted at 1:500.

REFERENCE
There are currently no references for CDK6 antibody [N3C3] (GTX113159). Be the first to share your publications with this product.
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169