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CDK7 antibody

IP/MS
Anti-CDK7 antibody used in Western Blot (WB). GTX111919
Anti-CDK7 antibody used in IHC (Paraffin sections) (IHC-P). GTX111919
Anti-CDK7 antibody used in Immunoprecipitation (IP). GTX111919

Cat. No. GTX111919

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CDK7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Product Note

IP/MS validation was supported by references (PMID:30377406)

Predict Reactivity

Mouse, Zebrafish, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human CDK7 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949952

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 7 , CAK , CAK1 , CDKN7 , HCAK , MO15 , STK1 , p39MO15

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDK7 antibody used in Western Blot (WB). GTX111919

GTX111919 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK7 antibody (GTX111919) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDK7 antibody used in IHC (Paraffin sections) (IHC-P). GTX111919

GTX111919 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CDK7(GTX111919) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CDK7 antibody used in Immunoprecipitation (IP). GTX111919

GTX111919 IP Image

CDK7 antibody immunoprecipitates CDK7 protein in IP experiments.
IP samples: 293T whole cell extract
A. 40 μg 293T whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of CDK7 protein by 4 μg CDK7 antibody (GTX111919)
10 % SDS-PAGE
The immunoprecipitated CDK7 protein was detected by CDK7 antibody (GTX111919) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

Application Reference

Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Narita T et al. Mol Cell Biol 2015; 35 (18) : 3178-88 Regulation of Transcription Elongation by the XPG-TFIIH Complex Is Implicated in Cockayne Syndrome.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169