CDK8 antibody [C3], C-term

Cat. No. GTX110495

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all CDK8 products

Datasheet
Document

View product citations for antibody GTX110495 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116

Predict Reactivity

Rat, Zebrafish, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CDK8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036538

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 8 , K35

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. [provided by RefSeq]

Database

Gene ID: 1024 CDK8
UniProt: P49336 CDK8

Research Area

DATA IMAGES

GTX110495 IHC-P Image

CDK8 antibody [C3], C-term detects CDK8 protein at cytoplasm and nucleus in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
CDK8 antibody [C3], C-term (GTX110495) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX110495 WB Image

CDK8 antibody detects CDK8 protein by western blot analysis. Various whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK8 antibody (GTX110495) diluted at a dilution of 1:3000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty