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CDK8 antibody

Cat. No. GTX22955

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
500 μl ($399)
See all CDK8 products

APPLICATION

Application Note

Immunofluorescence: assay dependent. Immunoprecipitation (with protein A, native): use at a concentration of 10μg / mg of lysate. Western Blotting: use at a concentration of 1 - 2μg / ml for 2hrs at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 54 kDa, which corresponds to the predicted molecular weight of Cdk 8.

Calculated MW

53 kDa. ( Note )

Positive Control

HT29 cells

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: C-SQQPPQYSHQTHRY, corresponding to amino acids 451-464 of Human CDK8.

Purification

Protein A affinity purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 8 , K35

Cellular Localization

Nucleus

Background

This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Database

Research Area

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399