GeneTex
United States (US)

CDK8 antibody

KO/KD
GTX33086_20180822_WB_18121410_852.jpg
GTX33086_20180530_WB_18121410_567.jpg

Cat No. GTX33086

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
See all CDK8 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 365-464 of human CDK8 (NP_001251.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cyclin Dependent Kinase 8,K35,Cdk8

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX33086_20180822_WB_18121410_852.jpg

GTX33086 WB Image

KO/KD

WB analysis of wild type and CDK8 knockout cell lysate using GTX33086 CDK8 antibody.
Dilution : 1:1000
Loading : 25μg per lane
GTX33086_20180530_WB_18121410_567.jpg

GTX33086 WB Image

WB analysis of Jurkat cell lysate using GTX33086 CDK8 antibody.
Dilution : 1:1000
Loading : 25μg per lane
Package List Price ($)
$ 329