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CDK8 antibody

KO/KD
Anti-CDK8 antibody used in Western Blot (WB). GTX33086
Anti-CDK8 antibody used in Immunoprecipitation (IP). GTX33086
Anti-CDK8 antibody used in Western Blot (WB). GTX33086

Cat. No. GTX33086

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399)
See all CDK8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 365-464 of human CDK8 (NP_001251.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 8 , K35

Cellular Localization

Nucleus

Background

This gene encodes a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit, cyclin C, are components of the Mediator transcriptional regulatory complex, involved in both transcriptional activation and repression by phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II. This kinase regulates transcription by targeting the cyclin-dependent kinase 7 subunits of the general transcription initiation factor IIH, thus providing a link between the Mediator complex and the basal transcription machinery. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-CDK8 antibody used in Western Blot (WB). GTX33086

GTX33086 WB Image

WB analysis of Jurkat cell lysate using GTX33086 CDK8 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CDK8 antibody used in Immunoprecipitation (IP). GTX33086

GTX33086 IP Image

IP analysis of Jurkat cell lysate using GTX33086 CDK8 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-CDK8 antibody used in Western Blot (WB). GTX33086

GTX33086 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX33086 CDK8 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399