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CDK9 antibody [N3C3]

Anti-CDK9 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107758
Anti-CDK9 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107758
Anti-CDK9 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107758
Anti-CDK9 antibody [N3C3] used in Western Blot (WB). GTX107758
Anti-CDK9 antibody [N3C3] used in Western Blot (WB). GTX107758

Cat. No. GTX107758

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CDK9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HL-60 , NIH-3T3

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDK9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166479

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase 9 , C-2k , CDC2L4 , CTK1 , PITALRE , TAK

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDK9 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107758

GTX107758 ICC/IF Image

CDK9 antibody [N3C3] detects CDK9 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDK9 protein stained by CDK9 antibody [N3C3] (GTX107758) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-CDK9 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107758

GTX107758 IHC-P Image

CDK9 antibody [N3C3] detects CDK9 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CDK9 antibody [N3C3] (GTX107758) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CDK9 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107758

GTX107758 IHC-P Image

CDK9 antibody [N3C3] detects CDK9 protein at cytoplasm and nucleus in rat adrenal gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
CDK9 antibody [N3C3] (GTX107758) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CDK9 antibody [N3C3] used in Western Blot (WB). GTX107758

GTX107758 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX107758 diluted at 1:1000

Anti-CDK9 antibody [N3C3] used in Western Blot (WB). GTX107758

GTX107758 WB Image

Sample (30 ug of whole cell lysate)
A: HL-60
10% SDS PAGE
GTX107758 diluted at 1:1000

REFERENCE

Application Reference

Choi HJ et al. EMBO Rep 2019; 20 (10) : e48058 CDK12 drives breast tumor initiation and trastuzumab resistance via WNT and IRS1-ErbB-PI3K signaling.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169