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CDKAL1 antibody

Anti-CDKAL1 antibody used in Western Blot (WB). GTX110020
Anti-CDKAL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110020
Anti-CDKAL1 antibody used in Western Blot (WB). GTX110020

Cat. No. GTX110020

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CDKAL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , NIH-3T3

Predict Reactivity

Zebrafish, Bovine, Cat, Dog, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDKAL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949953

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CDK5 regulatory subunit associated protein 1 like 1

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the methylthiotransferase family. The function of this gene is not known. Genome-wide association studies have linked single nucleotide polymorphisms in an intron of this gene with susceptibilty to type 2 diabetes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDKAL1 antibody used in Western Blot (WB). GTX110020

GTX110020 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX110020 diluted at 1:2000

Anti-CDKAL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110020

GTX110020 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using CDKAL1(GTX110020) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CDKAL1 antibody used in Western Blot (WB). GTX110020

GTX110020 WB Image

CDKAL1 antibody detects CDKAL1 protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg K562 whole cell lysate/extract
7.5 % SDS-PAGE
CDKAL1 antibody (GTX110020) dilution: 1:1000

REFERENCE

Application Reference

Brambillasca S et al. J Biol Chem 2012; 287 (50) : 41808-19 cdk5 regulatory subunit-associated protein 1-like 1 (cdkal1) is a tail-anchored protein in the endoplasmic reticulum (er) of *oma cells.
Application : WB
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169