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CDKN2A / p14ARF antibody

Cat. No. GTX11048

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Isotype

 IgG2a

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all CDKN2A / p14ARF products

APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 19 kDa. Optimal dilutions/concentrations should be determined by the end user.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

His-tagged CDKN2A / p14ARF protein expressed in bacteria.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_381228

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These two proteins interact with the upstream proteins: retinoblastoma protein and p53 tumor suppressors, respectively. Each of these proteins has a role in the senescence of primary cells, activates pathways for cell cycle control and tumor suppression. The p14ARF (or P19ARF in mouse) proteins can induce both G1 and G2 phase arrest in a manner that depends on functional p53. The mouse and human ARF proteins have different functions in tumor suppression; this distinction may contribute to the different levels of tumor proneness of these species.

Research Area

REFERENCE

Application Reference

Clark PA et al. Oncogene 2002; 21 (29) : 4498-507 Multiple interacting domains contribute to p14ARF mediated inhibition of MDM2.
Application : IP
Reactivity : Human
Llanos S et al. Nat Cell Biol 2001; 3 (5) : 445-52 Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolus.
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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations