APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2.18 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CDKN2A / p14ARF. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886118
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cyclin-dependent kinase inhibitor 2A Antibody , P16INK4 Antibody , TP16 Antibody , CDKN2 Antibody , MTS1 Antibody , P16 Antibody , P14ARF Antibody , CDKN2A Antibody , CMM2 Antibody , P16INK4A Antibody , MLM Antibody , ARF Antibody , INK4 Antibody , P19ARF Antibody , P19 Antibody , INK4A Antibody , P16-INK4A Antibody , CDK4I Antibody , P14 Antibody , MTS-1 Antibody
Background
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq]
Research Area
DATA IMAGES
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GTX129902 WB Image
Wild-type (WT) and p14ARF knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p14ARF antibody (GTX129902) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129902 IHC-P Image
p14ARF antibody detects p14ARF protein at nucleus in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
p14ARF antibody (GTX129902) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX129902 IHC-P Image
p14ARF antibody detects p14ARF protein at nucleus in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
p14ARF antibody (GTX129902) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX129902 IP Image
Immunoprecipitation of p14ARF protein from HeLa whole cell extracts using 5 μg of p14ARF antibody (GTX129902).
Western blot analysis was performed using p14ARF antibody (GTX129902).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX129902 WB Image
p14ARF antibody detects p14ARF protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with p14ARF antibody (GTX129902) at a dilution of 1:1000.
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REFERENCE
There are currently no references for CDKN2A / p14ARF antibody (GTX129902). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CDKN2A / p14ARF antibody (GTX129902). Be the first to share your experience with this product.
