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CDKN2A / p14ARF antibody

Anti-CDKN2A / p14ARF antibody used in Western Blot (WB). GTX20470
Anti-CDKN2A / p14ARF antibody used in Flow cytometry (FACS). GTX20470
Anti-CDKN2A / p14ARF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20470

Cat. No. GTX20470

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($449)
See all CDKN2A / p14ARF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:10 - 1:500
IHC-Fr 1:10 - 1:500
FACS 1:400
IP Assay dependent
ELISA 1:100 - 1:2000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to a portion of human p14ARF (between residues 50-150). [Swiss-Prot# Q8N726]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase inhibitor 2A , ARF , CDK4I , CDKN2 , CMM2 , INK4 , INK4A , MLM , MTS-1 , MTS1 , P14 , P14ARF , P16 , P16-INK4A , P16INK4 , P16INK4A , P19 , P19ARF , TP16

Cellular Localization

Cytoplasm,Nucleus,nucleoplasm,Nucleolus

Background

The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.

Database

Research Area

DATA IMAGES

Anti-CDKN2A / p14ARF antibody used in Western Blot (WB). GTX20470

GTX20470 WB Image

WB analysis of HeLa cell lysate using GTX20470 CDKN2A / p14ARF antibody.

Anti-CDKN2A / p14ARF antibody used in Flow cytometry (FACS). GTX20470

GTX20470 FACS Image

FACS analysis of HeLa cells using GTX20470 CDKN2A / p14ARF antibody. M1 is defined by unstained cells.
Purple : primary antibody
Dilution : 1:400

Anti-CDKN2A / p14ARF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX20470

GTX20470 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX20470 CDKN2A / p14ARF antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449