GeneTex
United States (US)

CDKN2A / p14ARF antibody

Overexpression
GTX30433_1398_WB_18121410_253.jpg
GTX30433_550_IHC-P_18121410_547.jpg

Cat No. GTX30433

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2500
IHC-P 1:500 - 1:1000
IHC-Fr 1:500 - 1:1000
ELISA 1:100 - 1:2000
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate pH 7.0 - 8.0, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The epitope recognized by this antibody maps to a region between residue 75 and 125 of human p14ARF using the numbering given in entry NP_478102.1 (GeneID 1029).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16, MTS 1, P16 INK4A

Background

The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.

Research Area

DATA IMAGES
GTX30433_1398_WB_18121410_253.jpg

GTX30433 WB Image

Overexpression

WB analysis of BT-549 (positive control), U2OS (negative control) and IMR90 overexpressing CDKN2A / p14ARF cell lysate using GTX30433 CDKN2A / p14ARF antibody.
Loading : 25μg
Dilution : 1 μg/ml

GTX30433_550_IHC-P_18121410_547.jpg

GTX30433 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX30433 CDKN2A / p14ARF antibody.
Dilution : 1 μg/ml