APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2500 |
| 1:100 - 1:500 |
| 1:100 - 1:2000 |
Not tested in other applications.
Predict Reactivity
Marmoset, Orangutan(>80% identity)
PROPERTIES
Form
Liquid
Buffer
Tris-Citrate/Phosphate
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The epitope recognized by this antibody maps to a region between residue 125 and the C-terminus (residue 173) of human p14ARF using the numbering given in entry NP_478102.1 (GeneID 1029).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cyclin dependent kinase inhibitor 2A , ARF , CDK4I , CDKN2 , CMM2 , INK4 , INK4A , MLM , MTS-1 , MTS1 , P14 , P14ARF , P16 , P16-INK4A , P16INK4 , P16INK4A , P19 , P19ARF , TP16
Cellular Localization
Cytoplasm,Nucleus,nucleoplasm,Nucleolus
Background
The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.
Database
Research Area
DATA IMAGES
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GTX30434 WB Image
WB analysis of BT-549 (positive control), U2OS (negative control) and IMR90 overexpressing CDKN2A / p14ARF cell lysate using GTX30434 CDKN2A / p14ARF antibody.
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GTX30434 IHC-P Image
IHC-P analysis of human prostate adenocarcinoma tissue using GTX30434 CDKN2A / p14ARF antibody.
Dilution : 1:250
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GTX30434 IHC-P Image
IHC-P analysis of human prostate carcinoma tissue using GTX30434 CDKN2A / p14ARF antibody.
Dilution : 1 μg/ml
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REFERENCE
There are currently no references for CDKN2A / p14ARF antibody (GTX30434). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CDKN2A / p14ARF antibody (GTX30434). Be the first to share your experience with this product.
