GeneTex
United States (US)

CDKN2A / p14ARF antibody

Overexpression
GTX30434_551_IHC-P_18121410_715.jpg
GTX30434_552_IHC-P_18121410_895.jpg
GTX30434_1399_WB_18121410_595.jpg

Cat No. GTX30434

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2500
IHC-P 1:100 - 1:500
ELISA 1:100 - 1:2000
Not tested in other applications.

Predict Reactivity

Marmoset, Orangutan(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris-Citrate/Phosphate pH 7.0 - 8.0, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The epitope recognized by this antibody maps to a region between residue 125 and the C-terminus (residue 173) of human p14ARF using the numbering given in entry NP_478102.1 (GeneID 1029).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16, MTS 1, P16 INK4A

Background

The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.

Research Area

DATA IMAGES
GTX30434_551_IHC-P_18121410_715.jpg

GTX30434 IHC-P Image

IHC-P analysis of human prostate adenocarcinoma tissue using GTX30434 CDKN2A / p14ARF antibody.
Dilution : 1:250

GTX30434_552_IHC-P_18121410_895.jpg

GTX30434 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX30434 CDKN2A / p14ARF antibody.
Dilution : 1 μg/ml

GTX30434_1399_WB_18121410_595.jpg

GTX30434 WB Image

Overexpression

WB analysis of BT-549 (positive control), U2OS (negative control) and IMR90 overexpressing CDKN2A / p14ARF cell lysate using GTX30434 CDKN2A / p14ARF antibody.