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CDKN2A / p16INK4a antibody [1D7D2]

Anti-CDKN2A / p16INK4a antibody [1D7D2] used in Flow cytometry (FACS). GTX60663
Anti-CDKN2A / p16INK4a antibody [1D7D2] used in IHC (Paraffin sections) (IHC-P). GTX60663
Anti-CDKN2A / p16INK4a antibody [1D7D2] used in IHC (Paraffin sections) (IHC-P). GTX60663
Anti-CDKN2A / p16INK4a antibody [1D7D2] used in ELISA (ELISA). GTX60663
Anti-CDKN2A / p16INK4a antibody [1D7D2] used in Western Blot (WB). GTX60663

Cat. No. GTX60663

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1D7D2

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all CDKN2A / p16INK4a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CDKN2A (AA: 1-156) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase inhibitor 2A , ARF , CDK4I , CDKN2 , CMM2 , INK4 , INK4A , MLM , MTS-1 , MTS1 , P14 , P14ARF , P16 , P16-INK4A , P16INK4 , P16INK4A , P19 , P19ARF , TP16

Cellular Localization

Cytoplasm,Nucleus,nucleoplasm,Nucleolus

Background

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]

Database

Research Area

DATA IMAGES

Anti-CDKN2A / p16INK4a antibody [1D7D2] used in Flow cytometry (FACS). GTX60663

GTX60663 FACS Image

FACS analysis of HEK293 cells using GTX60663 CDKN2A / p16INK4a antibody [1D7D2].
Green : CDKN2A / p16INK4a
Red : negative control

Anti-CDKN2A / p16INK4a antibody [1D7D2] used in IHC (Paraffin sections) (IHC-P). GTX60663

GTX60663 IHC-P Image

IHC-P analysis of endometrial cancer tissue using GTX60663 CDKN2A / p16INK4a antibody [1D7D2].

Anti-CDKN2A / p16INK4a antibody [1D7D2] used in IHC (Paraffin sections) (IHC-P). GTX60663

GTX60663 IHC-P Image

IHC-P analysis of lung cancer tissue using GTX60663 CDKN2A / p16INK4a antibody [1D7D2].

Anti-CDKN2A / p16INK4a antibody [1D7D2] used in ELISA (ELISA). GTX60663

GTX60663 ELISA Image

ELISA analysis of antigen using GTX60663 CDKN2A / p16INK4a antibody [1D7D2].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-CDKN2A / p16INK4a antibody [1D7D2] used in Western Blot (WB). GTX60663

GTX60663 WB Image

WB analysis of HEK293 (1) and CDKN2A (AA: 1-156)-hIgGFc transfected HEK293 (2) cell lysate using GTX60663 CDKN2A / p16INK4a antibody [1D7D2].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399