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CDKN2A / p16INK4a antibody [GT888]

Anti-CDKN2A / p16INK4a antibody [GT888] used in Western Blot (WB). GTX635639

Cat No. GTX635639

Host

Mouse

Clonality

Monoclonal

Clone Name

GT888

Isotype

IgG1

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

293T , HeLa

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CDKN2A / p16INK4a. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cyclin dependent kinase inhibitor 2A , ARF , CDK4I , CDKN2 , CMM2 , INK4 , INK4A , MLM , MTS-1 , MTS1 , P14 , P14ARF , P16 , P16-INK4A , P16INK4 , P16INK4A , P19 , P19ARF , TP16

Cellular Localization

Cytoplasm,Nucleus,nucleoplasm,Nucleolus

Background

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]

Database

Research Area

DATA IMAGES

Anti-CDKN2A / p16INK4a antibody [GT888] used in Western Blot (WB). GTX635639

GTX635639 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CDKN2A / p16INK4a antibody [GT888] (GTX635639) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

There are currently no references for CDKN2A / p16INK4a antibody [GT888] (GTX635639). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CDKN2A / p16INK4a antibody [GT888] (GTX635639). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 329
$ 169