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CDKN2A / p16INK4a antibody

Anti-CDKN2A / p16INK4a antibody used in Western Blot (WB). GTX03119
Anti-CDKN2A / p16INK4a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03119
Anti-CDKN2A / p16INK4a antibody used in Immunoprecipitation (IP). GTX03119
Anti-CDKN2A / p16INK4a antibody used in IHC (Paraffin sections) (IHC-P). GTX03119
Anti-CDKN2A / p16INK4a antibody used in IHC (Paraffin sections) (IHC-P). GTX03119
Anti-CDKN2A / p16INK4a antibody used in IHC (Paraffin sections) (IHC-P). GTX03119

Cat. No. GTX03119

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CDKN2A / p16INK4a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
IP 1:50-1:200
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 20 to the C-terminus of human CDKN2A / p16INK4a.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase inhibitor 2A , ARF , CDK4I , CDKN2 , CMM2 , INK4 , INK4A , MLM , MTS-1 , MTS1 , P14 , P14ARF , P16 , P16-INK4A , P16INK4 , P16INK4A , P19 , P19ARF , TP16

Cellular Localization

Cytoplasm , Nucleus , nucleoplasm , Nucleolus

Background

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]

Database

Research Area

DATA IMAGES

Anti-CDKN2A / p16INK4a antibody used in Western Blot (WB). GTX03119

GTX03119 WB Image

WB analysis of HeLa cell lysate using GTX03119 CDKN2A / p16INK4a antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CDKN2A / p16INK4a antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03119

GTX03119 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX03119 CDKN2A / p16INK4a antibody.
Blue : DAPI for nuclear staining
Dilution : 1:100

Anti-CDKN2A / p16INK4a antibody used in Immunoprecipitation (IP). GTX03119

GTX03119 IP Image

IP analysis of HeLa cell lysate using GTX03119 CDKN2A / p16INK4a antibody.
Antibody amount : 3μg / 300μg extracts
Dilution : 1:1000

Anti-CDKN2A / p16INK4a antibody used in IHC (Paraffin sections) (IHC-P). GTX03119

GTX03119 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX03119 CDKN2A / p16INK4a antibody.
Dilution : 1:100

Anti-CDKN2A / p16INK4a antibody used in IHC (Paraffin sections) (IHC-P). GTX03119

GTX03119 IHC-P Image

IHC-P analysis of rat liver tissue using GTX03119 CDKN2A / p16INK4a antibody.
Dilution : 1:100

Anti-CDKN2A / p16INK4a antibody used in IHC (Paraffin sections) (IHC-P). GTX03119

GTX03119 IHC-P Image

IHC-P analysis of human colon tissue using GTX03119 CDKN2A / p16INK4a antibody.
Dilution : 1:100

REFERENCE

Application Reference

Yu R et al. Int J Biol Sci 2022; 18 (9) : 3874-3887 Integrative Analyses of m6A Regulators Identify that METTL3 is Associated with HPV Status and Immunosuppressive Microenvironment in HPV-related Cancers.
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REVIEW

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Package List Price ($)
$ 399