GeneTex

  • Country / Location Selection

United States (US)

CDON / CDO antibody [N1N2], N-term

Anti-CDON / CDO antibody [N1N2], N-term used in Western Blot (WB). GTX105422
Anti-CDON / CDO antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX105422
Anti-CDON / CDO antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX105422

Cat. No. GTX105422

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CDON / CDO products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , SY-SY5Y

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CDO. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036541

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cell adhesion associated, oncogene regulated , CDO , CDON1 , HPE11 , ORCAM

Cellular Localization

Membrane

Background

CDON and BOC (MIM 608708) are cell surface receptors of the immunoglobulin (Ig)/fibronectin type III (FNIII; see MIM 135600) repeat family involved in myogenic differentiation. CDON and BOC are coexpressed during development, form complexes with each other in a cis fashion, and are related to each other in their ectodomains, but each has a unique long cytoplasmic tail.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CDON / CDO antibody [N1N2], N-term used in Western Blot (WB). GTX105422

GTX105422 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CDO antibody [N1N2], N-term (GTX105422) diluted at 1:500.

Anti-CDON / CDO antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX105422

GTX105422 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using CDO(GTX105422) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CDON / CDO antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX105422

GTX105422 IHC-P Image

The data was published in the journal Nat Commun in 2020.PMID: 32332736

REFERENCE

Application Reference

Lee J et al. Sci Rep 2023; 13 (1) : 12309 Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development.
Kim Y et al. Nat Commun 2020; 11 (1) : 1994 Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration.
Application : IHC-P
Reactivity : Mouse
Lu IL et al. Nat Commun 2018; 9 (1) : 2498 Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for CDON / CDO antibody [N1N2], N-term (GTX105422). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169