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CDR2 antibody

Anti-CDR2 antibody used in Western Blot (WB). GTX129122
Anti-CDR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129122
Anti-CDR2 antibody used in Immunoprecipitation (IP). GTX129122
Anti-CDR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129122

Cat No. GTX129122

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDR2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cerebellar degeneration related protein 2 , CDR62 , Yo

Database

Research Area

DATA IMAGES

Anti-CDR2 antibody used in Western Blot (WB). GTX129122

GTX129122 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDR2 antibody (GTX129122) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDR2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129122

GTX129122 ICC/IF Image

CDR2 antibody detects CDR2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CDR2 protein stained by CDR2 antibody (GTX129122) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-CDR2 antibody used in Immunoprecipitation (IP). GTX129122

GTX129122 IP Image

CDR2 antibody immunoprecipitates CDR2 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of CDR2 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of CDR2 by 2.5 μg of CDR2 antibody (GTX129122) 10% SDS-PAGE The immunoprecipitated CDR2 protein was detected by CDR2 antibody (GTX129122) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-CDR2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129122

GTX129122 IHC-P Image

CDR2 antibody detects CDR2 protein on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
CDR2 antibody (GTX129122) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169