APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:200-1:2000 |
1:100-1:1000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Serum
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
E. coli expressed and purified 6xHis-tagged C-terminal 150 amino acids of human Cdt2
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CDT2 , DCAF2 , DTL , L2DTL , RAMP , denticleless E3 ubiquitin protein ligase homolog
Cellular Localization
Nucleus,Nucleus membrane,Cytoplasm,Cytoskeleton,Microtubule organizing center,Centrosome,Chromosome
Database
Research Area
DATA IMAGES
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GTX00893 WB Image
WB analysis of HeLa cells were transfected with control or Cdt2 siRNA using GTX00893 CDT2 antibody. Cdt2 is phosphorylated after UV irradiation as shown by the band shift-up in irradiated sample (UV +).
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GTX00893 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX00893 CDT2 antibody. Cdt2 protein is localized in nuclei at all cell stages. Fixation : 4% PFA, 10 min Permeabilization : 0.25% (v/v) Triton X-100 in PBS
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GTX00893 WB Image
WB analysis of HeLa whole cell lysate using GTX00893 CDT2 antibody. Dilution : 1:2000 Loading : 20 μg
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GTX00893 WB Image
WB analysis of various samples using GTX00893 CDT2 antibody. Doublet bands reflect post-translational phospholylation. Lane 1 : HeLa whole cell lysate Lane 2 : MCF-7 whole cell lysate Lane 3 : NIH-3T3 whole cell lysate Lane 4 : CHO whole cell lysate Dilution : 1:200 Loading : 40 μg
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REFERENCE
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REVIEW
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