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CDX2 antibody

Anti-CDX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113160
Anti-CDX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113160
Anti-CDX2 antibody used in Western Blot (WB). GTX113160

Cat No. GTX113160

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

HCT116 , HCT116 nuclear extract

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CDX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168978

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

caudal type homeobox 2 , CDX-3 , CDX2/AS , CDX3

Cellular Localization

Nucleus

Background

The level and beta-cell specificity of insulin gene expression are regulated by a set of nuclear proteins that bind to specific sequences within the promoter of the insulin gene (INS; MIM 176730) and interact with RNA polymerase to activate or repress transcription. The proteins LMX1 (MIM 600298) and CDX3 are homeodomain proteins that bind an A/T-rich sequence in the insulin promoter and stimulate its transcription (German et al., 1994 [PubMed 7698771]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CDX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113160

GTX113160 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CDX2(GTX113160) antibody at 1:500 dilution.

Anti-CDX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113160

GTX113160 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human normal colon, using CDX2(GTX113160) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CDX2 antibody used in Western Blot (WB). GTX113160

GTX113160 WB Image

HCT116 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDX2 antibody (GTX113160) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

CDX2 antibody Cat No. GTX113160

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 20-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : CaCo2 cells
Amount used : 10 μg
Blocking : 0.5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : Undiluted, 25°C, 12Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169