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CDYL antibody

Orthogonal
Anti-CDYL antibody used in Western Blot (WB). GTX117708
Anti-CDYL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117708
Anti-CDYL antibody used in IHC (Paraffin sections) (IHC-P). GTX117708

Cat. No. GTX117708

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all CDYL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

MDA-MB-341 nuclear extracts

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CDYL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166284

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromodomain Y like , CDYL1

Cellular Localization

Nucleus

Background

Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CDYL antibody used in Western Blot (WB). GTX117708

GTX117708 WB Image

Orthogonal

MDA-MB-231 whole cell and nuclear extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDYL antibody (GTX117708) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-CDYL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117708

GTX117708 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CDYL(GTX117708) antibody at 1:500 dilution.

Anti-CDYL antibody used in IHC (Paraffin sections) (IHC-P). GTX117708

GTX117708 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using CDYL(GTX117708) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for CDYL antibody (GTX117708). Be the first to share your publications with this product.
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REVIEW

CDYL antibody Cat. No. GTX117708

Rating
( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
Anti-CDYL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117708
Date : Anonymous submitted on 22-Aug-2013
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : mouse kidney
Blocking : 10% normal goat serum, 25°C, 0.5Hr
Fixation : 4℅ paraformaldehyde in PBS
Permeabilization /Antigen retrieval : 1X PBS containing 0.4% Triton X-100 and 2% BSA
Primary Antibody : 1:100, 4°C, 16Hr
Figure Note : Red:CDYL, Blue:DAPI, CDYL is also expressed in cytoplasm.(PMID:20402673)
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169