CDw75 antibody [FR9]

Cat. No. GTX04883

Host	Mouse
Clonality	Monoclonal
Clone Name	FR9
Isotype	IgM
Application	WB, ICC/IF, FACS, ELISA
Package	100 μg ($599)

See all CDw75 products

Datasheet

View product citations for antibody GTX04883 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:250-1:1000
ICC/IF	Assay dependent
FACS	Assay dependent
ELISA	Assay dependent
Note : WB Skim milk is not recommended for blocking.

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Product Note

This antibody is specific for CDw75 (Neu5Acα2,6Galβ1,4GlcNAc) in glycoproteins and glycolipids expressed in mammalian cells and does not cross react with other similar glycan structures: LN, 3SLN, GM3, GD1a, GT1b, and asialofetuin, determined by ELISA assay. (PMID: 33564002)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CDw75-labeled ceramide lipid (C12L)

Purification

Purified by DEAE ion exchange chromatography followed by hydroxyapatite chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SIAT1,ST6 betagalactoside alpha2,6sialyltransferase 1,ST6GAL1,ST6GalI,ST6N,Cdw75,ST6 beta-galactoside alpha-2,6-sialyltransferase 1

Cellular Localization

Golgi apparatus,Golgi stack membrane,Secreted

Background

This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]

Database

Gene ID: 6480 ST6GAL1
UniProt: P15907 ST6GAL1

Research Area

DATA IMAGES

GTX04883 ELISA Image

ELISA analysis of Fetuin using serially diluted GTX04883 CDw75 antibody [FR9].

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 599

Qty