GeneTex
United States (US)

CEA antibody [1106]

GTX15677_1516_WB_19011519_647.jpg
GTX15677_105_FACS_19011519_636.jpg
GTX15677_287_ICC-IF_19011519_575.jpg

Cat No. GTX15677

Host Mouse
Clonality Monoclonal
Clone Name 1106
Isotype IgG1
Application WB, ICC/IF, FACS, IP, ELISA, RIA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1μg/ml
ICC/IF Assay dependent
FACS 5-20μg/ml
IP Assay dependent
ELISA Assay dependent
RIA Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

0.01M potassium phosphate, 0.135M NaCl, 2.5mM KCl, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified CEA from pooled tumors

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immun

Research Area

DATA IMAGES
GTX15677_1516_WB_19011519_647.jpg

GTX15677 WB Image

WB analysis of 20 ug of BxPC-3, HeLa, and HepG2 whole cell lysates per well using GTX15677 CEA antibody [1106].
Dilution : 1 µg/ml

GTX15677_105_FACS_19011519_636.jpg

GTX15677 FACS Image

FACS analysis of BxPC-3 cells using GTX15677 CEA antibody [1106].
Pink histogram : Primary antibody
Black histogram : Isotype control antibody
Dilution : 10 µg/ml in PBS + 5% FCS

GTX15677_287_ICC-IF_19011519_575.jpg

GTX15677 ICC/IF Image

ICC/IF analysis of BxPC-3 cells using GTX15677 CEA antibody [1106].
Green : Primary antibody
Blue : Nuclei
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
Dilution : 10 µg/ml in blocking buffer for 1 hour at room temperature