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CENPF antibody [14C10 1D8]

Anti-CENPF antibody [14C10 1D8] used in IHC (Paraffin sections) (IHC-P). GTX70137
Anti-CENPF antibody [14C10 1D8] used in Western Blot (WB). GTX70137
Anti-CENPF antibody [14C10 1D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70137

Cat No. GTX70137

Host

Mouse

Clonality

Monoclonal

Clone Name

14C10 1D8

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

350 kDa. ( Note )

Positive Control

HeLa (G2 phase)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Centromere Protein F , Cenf , Cild31 , Pro1779 , Stroms , Hcp-1 , Cenpf

Cellular Localization

Nuclear

Background

Mitosin-14C10 recognizes mitosin, a 350kDa phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase, and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.

Database

Research Area

DATA IMAGES

Anti-CENPF antibody [14C10 1D8] used in IHC (Paraffin sections) (IHC-P). GTX70137

GTX70137 IHC-P Image

CENPF antibody [14C10 1D8] detects CENPF protein at nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
CENPF antibody [14C10 1D8] (GTX70137) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CENPF antibody [14C10 1D8] used in Western Blot (WB). GTX70137

GTX70137 WB Image

CENPF antibody [14C10 1D8] detects CENPF protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
5% SDS-PAGE
CENPF antibody [14C10 1D8] (GTX70137) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CENPF antibody [14C10 1D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70137

GTX70137 ICC/IF Image

Confocal immunofluorescence staining (Olympus FV10i) of Mitosin. U2OS cells were fixed by 4% PFA and costained with Mitosin 14C10 1D8 AB (Red; cat# GTX70137; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# GTX112141; 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329