CENPF antibody [14C10 1D8]

Cat. No. GTX70137
9
Cat. No.  GTX70137
Orthogonal Carrier-Free
Anti-CENPF antibody [14C10 1D8] used in IHC (Paraffin sections) (IHC-P). GTX70137
GTX70137 IHC-P Image

CENPF antibody [14C10 1D8] detects CENPF protein at nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
CENPF antibody [14C10 1D8] (GTX70137) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-CENPF antibody [14C10 1D8] used in Western Blot (WB). GTX70137
GTX70137 WB Image
Orthogonal

CENPF antibody [14C10 1D8] detects CENPF protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
5% SDS-PAGE
CENPF antibody [14C10 1D8] (GTX70137) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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Anti-CENPF antibody [14C10 1D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70137
GTX70137 ICC/IF Image

Confocal immunofluorescence staining (Olympus FV10i) of Mitosin. U2OS cells were fixed by 4% PFA and costained with Mitosin 14C10 1D8 AB (Red; cat# GTX70137; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# GTX112141; 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um

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Anti-CENPF antibody [14C10 1D8] used in IHC (Paraffin sections) (IHC-P). GTX70137
Anti-CENPF antibody [14C10 1D8] used in Western Blot (WB). GTX70137
Anti-CENPF antibody [14C10 1D8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70137
This CENPF antibody [14C10 1D8] (GTX70137) is a mouse monoclonal antibody validated for detection of CENPF by WB, ICC/IF, IHC-P, IHC-Fr, FCM, and IP. Reactivity has been shown in Human.
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    14C10 1D8
  • Isotype
    IgG2a
  • Applications
    WB ICC/IF IHC-P IHC-Fr FCM IP
  • Reactivity
    Human
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