CENPF antibody [14C10 1D8]

Cat. No. GTX70137

Reference 7

Host	Mouse
Clonality	Monoclonal
Clone Name	14C10 1D8
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP
Reactivity	Human
Package	100 μl ($399)

See all CENPF products

View product citations for antibody GTX70137 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500
ICC/IF	Assay dependent
IHC-P	Assay dependent
IHC-Fr	Assay dependent
FACS	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW

358 kDa. ( Note )

Positive Control

HeLa (G2 phase)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_369386

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centromere protein F , CENF , CILD31 , PRO1779 , STROMS , hcp-1

Background

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]

Database

Gene ID: 1063 CENPF
UniProt: P49454 CENPF

Research Area

DATA IMAGES

GTX70137 ICC/IF Image

Confocal immunofluorescence staining (Olympus FV10i) of Mitosin. U2OS cells were fixed by 4% PFA and costained with Mitosin 14C10 1D8 AB (Red; cat# GTX70137; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# GTX112141; 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um

GTX70137 WB Image

CENPF antibody [14C10 1D8] detects CENPF protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
5% SDS-PAGE
CENPF antibody [14C10 1D8] (GTX70137) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70137 IHC-P Image

CENPF antibody [14C10 1D8] detects CENPF protein at nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
CENPF antibody [14C10 1D8] (GTX70137) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min