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CENPF antibody [7F11.2]

Anti-CENPF antibody [7F11.2] used in Immunohistochemistry (IHC). GTX77645
Anti-CENPF antibody [7F11.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX77645

Cat. No. GTX77645

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7F11.2

Isotype

 IgG2a

Application

 ICC/IF, IHC

Reactivity

 Human
Package
100 μl ($399)
See all CENPF products

APPLICATION

Application Note

For IHC: Use at a concentration of 10 g/mL. Briefly, 4-m tissue sections were cut from the same blocks of hematoxylin and eosin-stained slides, mounted on charged slides, deparaffinized in xylene, and rehydrated in descending grades of ethyl alcohol (from 100% to 70%). The slides used for mitosin staining were then subjected to heat-induced antigen retrieval by immersion in a 0.01 mol/L citrate buffer, pH 6.0; preheated to > 90 ¡C; and heated in an electric vegetable steamer (Black and Decker, Shelton, CT) for 54 minutes. The endogenous peroxidase activity was blocked by a 5-minute treatment with 3% hydrogen peroxide in absolute methanol. Immunohistochemical analysis was then performed using the avidin-biotin technique (LSAB2 peroxidase kit; DAKO, Carpinteria, CA). The antigen-antibody immunoreaction was visualized using 3-3-diaminobenzedine as the chromogen, and the slides were counterstained with Mayer hematoxylin. For ICC/IF: Use at a dilution of 1:500. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

358 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_424593

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centromere protein F , CENF , CILD31 , PRO1779 , STROMS , hcp-1

Background

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CENPF antibody [7F11.2] used in Immunohistochemistry (IHC). GTX77645

GTX77645 IHC Image

High magnification photomicrograph of an NG3 tumor with a high percentage of mitosin-positive nuclei, indicative of high proliferative activity.

Anti-CENPF antibody [7F11.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX77645

GTX77645 ICC/IF Image

Immunofluorescent staining of mitotic U2OS cells with mitosin/CENP-F antibody GTX77645 (green) at 1:500 dilution. Cells were pre-extracted with ice-cold CSK buffer (0.5% Triton X-100, 300 mM Sucrose, 10 mM PIPES, pH8.0) for 4 min, followed by fixation with 4% PFA and regular IFA procedure. Blue channel (DAPI staining)
shows chromosomes.

REFERENCE

Application Reference

Wang J et al. Cancer 2002; 94 (12) : 3107-14 Assessment of histologic features and expression of biomarkers in predicting pathologic response to anthracycline-based neoadjuvant chemotherapy in patients with breast carcinoma.
Application : IHC
Reactivity : Human
Submit a Reference

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Package List Price ($)
$ 399
Custom Conjugations