Immunofluorescent staining of mitotic U2OS cells with mitosin/CENP-F antibody GTX77645 (green) at 1:500 dilution. Cells were pre-extracted with ice-cold CSK buffer (0.5% Triton X-100, 300 mM Sucrose, 10 mM PIPES, pH8.0) for 4 min, followed by fixation with 4% PFA and regular IFA procedure. Blue channel (DAPI staining)
shows chromosomes.

High magnification photomicrograph of an NG3 tumor with a high percentage of mitosin-positive nuclei, indicative of high proliferative activity.