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CENPF antibody [C3], C-term

Anti-CENPF antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100212
Anti-CENPF antibody [C3], C-term used in Western Blot (WB). GTX100212
Anti-CENPF antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100212
Anti-CENPF antibody [C3], C-term used in IHC (Whole mount) (IHC-Wm). GTX100212

Cat. No. GTX100212

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all CENPF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

368 kDa. ( Note )

Positive Control

Raji

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CENPF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949961

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centromere protein F , CENF , CILD31 , PRO1779 , STROMS , hcp-1

Cellular Localization

Cytoplasm , perinuclear region , Nucleus matrix , Kinetochore , Spindle

Background

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CENPF antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100212

GTX100212 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using CENPF(GTX100212) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CENPF antibody [C3], C-term used in Western Blot (WB). GTX100212

GTX100212 WB Image

Sample (30 μg of whole cell lysate)
A: Raji
5% SDS PAGE
GTX100212 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CENPF antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100212

GTX100212 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using CENPF(GTX100212) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-CENPF antibody [C3], C-term used in IHC (Whole mount) (IHC-Wm). GTX100212

GTX100212 IHC-Wm Image

The data was published in the journal Oncotarget in 2014.PMID: 25051368

REFERENCE

Application Reference

Li JN et al. Cancers (Basel) 2021; 13 (24) Expression of SnoRNA U50A Is Associated with Better Prognosis and Prolonged Mitosis in Breast Cancer.
Chen CH et al. Oncotarget 2014; (Epub) Cdc6 cooperates with c-Myc to promote genome instability and EMT in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169