GeneTex
United States (US)

CENPF antibody [C3], C-term

GTX100212_39391_IHC_19011506_821.jpg
GTX100212_39391_IFA_19011506_539.jpg
GTX100212_WB_19011506_297.jpg

Cat No. GTX100212

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Wm
Reactivity Human, Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

368 kDa. ( Note )

Positive Control

293T , Raji
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CENPF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CENF antibody, PRO1779 antibody, hcp-1 antibody, CENPF antibody, CENP-F kinetochore protein antibody, kinetochore protein CENPF antibody, centromere protein F antibody, mitosin antibody, cell-cycle-dependent 350K nuclear protein antibody, "centromere protein F, 350/400ka (mitosin) antibody", AH antigen antibody, "centromere protein F, 350/400kDa (mitosin) antibody"

Cellular Localization

Cytoplasm , perinuclear region , Nucleus matrix , Kinetochore , Spindle

Background

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100212_39391_IHC_19011506_821.jpg

GTX100212 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using CENPF(GTX100212) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100212_39391_IFA_19011506_539.jpg

GTX100212 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using CENPF(GTX100212) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

GTX100212_WB_19011506_297.jpg

GTX100212 WB Image

Sample (30 µg of whole cell lysate)
A: Raji
5% SDS PAGE
GTX100212 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169