GeneTex
United States (US)

CENPF antibody

GTX30232_196_ICC-IF_18121410_738.jpg
GTX30232_1369_WB_18121410_648.jpg
GTX30232_197_ICC-IF_18121410_847.jpg

Cat No. GTX30232

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS, IP, WB-Ag
Reactivity Human, Bovine
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1500 - 1:2500
ICC/IF 1:200 - 1:1000
FACS 1:50 - 1:200
IP 1:50
WB-Ag Assay dependent
Not tested in other applications.

Calculated MW

368 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A bacterial fusion protein from the C-terminus of human CENPF. [UniProt# P49454]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Centromere Protein F,Cenf,Cild31,Pro1779,Stroms,Hcp-1,Cenpf

Cellular Localization

Cytoplasm,perinuclear region,Nucleus matrix,Kinetochore,Spindle

Background

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX30232_196_ICC-IF_18121410_738.jpg

GTX30232 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30232 CENPF antibody.
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:5

GTX30232_1369_WB_18121410_648.jpg

GTX30232 WB Image

WB analysis of asynchronous and mitotically blocked HeLa lysates using GTX30232 CENPF antibody.
Loading : 25μg

GTX30232_197_ICC-IF_18121410_847.jpg

GTX30232 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30232 CENPF antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

REFERENCE
Package List Price ($)
$ 369