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CENPK antibody

Anti-CENPK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65896
Anti-CENPK antibody used in Western Blot (WB). GTX65896

Cat. No. GTX65896

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all CENPK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-269 of human CENPK (NP_071428.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centromere protein K , AF5alpha , CENP-K , FKSG14 , P33 , Solt

Cellular Localization

Nucleus

Background

CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-CENPK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65896

GTX65896 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX65896 CENPK antibody.
Blue : DAPI
Dilution : 1:100

Anti-CENPK antibody used in Western Blot (WB). GTX65896

GTX65896 WB Image

WB analysis of various sample lysates using GTX65896 CENPK antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399