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CEP152 antibody [HL2763]

Recombinant antibody Orthogonal
Anti-CEP152 antibody [HL2763] used in Western Blot (WB). GTX639628
Anti-CEP152 antibody [HL2763] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639628

Cat. No. GTX639628

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2763

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CEP152 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

196 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human CEP152.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centrosomal protein 152 , MCPH4 , MCPH9 , SCKL5

Cellular Localization

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Background

This gene encodes a protein that is thought to be involved with centrosome function. Mutations in this gene have been associated with primary microcephaly (MCPH4). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-CEP152 antibody [HL2763] used in Western Blot (WB). GTX639628

GTX639628 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CEP152 antibody [HL2763] (GTX639628) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CEP152 antibody [HL2763] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX639628

GTX639628 ICC/IF Image

CEP152 antibody [HL2763] detects CEP152 protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CEP152 stained by CEP152 antibody [HL2763] (GTX639628) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations