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CEP152 antibody

Anti-CEP152 antibody used in Western Blot (WB). GTX128027
Anti-CEP152 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128027
Anti-CEP152 antibody used in Immunoprecipitation (IP). GTX128027

Cat No. GTX128027

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP, IHC

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

189 kDa. ( Note )

Positive Control

293T , HeLa

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CEP152. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

centrosomal protein 152 , MCPH4 , MCPH9 , SCKL5

Cellular Localization

Centrosome

Background

This gene encodes a protein that is thought to be involved with centrosome function. Mutations in this gene have been associated with primary microcephaly (MCPH4). Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CEP152 antibody used in Western Blot (WB). GTX128027

GTX128027 WB Image

CEP152 antibody detects CEP152 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
5 % SDS-PAGE
CEP152 antibody (GTX128027) dilution: 1:1000

Anti-CEP152 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128027

GTX128027 ICC/IF Image

CEP152 antibody detects CEP152 protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: CEP152 protein stained by CEP152 antibody (GTX128027) diluted at 1:2000.
Red: Tubulin, alpha 1b protein stained by TUBA1B antibody (GTX11304) diluted at 1:5000.
Blue: Hoechst 33342 staining.

Anti-CEP152 antibody used in Immunoprecipitation (IP). GTX128027

GTX128027 IP Image

Immunoprecipitation of CEP152 protein from HeLa whole cell extracts using 5 μg of CEP152 antibody (GTX128027).
Western blot analysis was performed using CEP152 antibody (GTX128027).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169