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CEP152 antibody

Orthogonal
Anti-CEP152 antibody used in Western Blot (WB). GTX128027
Anti-CEP152 antibody used in Immunoprecipitation (IP). GTX128027
Anti-CEP152 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128027
Anti-CEP152 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128027

Cat. No. GTX128027

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IP, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CEP152 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

189 kDa. ( Note )

Positive Control

293T , HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CEP152. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885681

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centrosomal protein 152 , MCPH4 , MCPH9 , SCKL5

Cellular Localization

Centrosome

Background

This gene encodes a protein that is thought to be involved with centrosome function. Mutations in this gene have been associated with primary microcephaly (MCPH4). Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CEP152 antibody used in Western Blot (WB). GTX128027

GTX128027 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CEP152 antibody (GTX128027) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CEP152 antibody used in Immunoprecipitation (IP). GTX128027

GTX128027 IP Image

Immunoprecipitation of CEP152 protein from HeLa whole cell extracts using 5 μg of CEP152 antibody (GTX128027).
Western blot analysis was performed using CEP152 antibody (GTX128027).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CEP152 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128027

GTX128027 ICC/IF Image

CEP152 antibody detects CEP152 protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: CEP152 protein stained by CEP152 antibody (GTX128027) diluted at 1:2000.
Red: Tubulin, alpha 1b protein stained by TUBA1B antibody (GTX11304) diluted at 1:5000.
Blue: Hoechst 33342 staining.
Anti-CEP152 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128027

GTX128027 ICC/IF Image

CEP152 antibody detects CEP152 protein at cytoskeleton and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in MeOH.
Green: CEP152 stained by CEP152 antibody (GTX128027) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Aziz K et al. J Clin Invest 2018; 128 (8) : 3517-3534 Mosaic-variegated aneuploidy syndrome mutation or haploinsufficiency in Cep57 impairs tumor suppression.
Application : IHC
Reactivity : Mouse
Submit a Reference

REVIEW

CEP152 antibody Cat. No. GTX128027

Rating
( Average 4 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 4 based on 1 users reviews)
Anti-CEP152 antibody used in IHC (Frozen sections) (IHC-Fr). GTX128027
Date : Anonymous submitted on 30-Oct-2023
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : E14.5 Mouse cortex tissue
Blocking : 0.1% BSA, 25°C, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.05% Triton in PBS
Primary Antibody : 1:100, 4°C, 18Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169