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CEP164 antibody [13]

Anti-CEP164 antibody [13] used in Western Blot (WB). GTX70094
Anti-CEP164 antibody [13] used in Western Blot (WB). GTX70094

Cat No. GTX70094

Host

Mouse

Clonality

Monoclonal

Clone Name

13

Isotype

IgG1

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

164 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human CEP164.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Centrosomal Protein 164 , Nphp15 , Cep164

Cellular Localization

Localizes specifically to very distally located appendage structures on the mature centriole from which initiate PC formation. Persisted at centrioles throughout mitosis. Expressed in chromatin-enriched nuclear fraction of HeLa cells.

Background

This monoclonal antibody binds to Cep164 or centrosonal protein 164kDa. Cep164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA,CHK2 and CHK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHK1. [from UniProt]

Database

Research Area

DATA IMAGES

Anti-CEP164 antibody [13] used in Western Blot (WB). GTX70094

GTX70094 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
5% SDS PAGE
GTX70094 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CEP164 antibody [13] used in Western Blot (WB). GTX70094

GTX70094 WB Image

Cep 164 antibody [13] detects Cep 164 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and blotted with Cep 164 antibody [13] (GTX70094) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329