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CEP164 antibody [17]

Anti-CEP164 antibody [17] used in Western Blot (WB). GTX70096
Anti-CEP164 antibody [17] used in Western Blot (WB). GTX70096

Cat No. GTX70096

Host

Mouse

Clonality

Monoclonal

Clone Name

17

Isotype

IgG1

Application

WB, IP

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
IP Assay dependent
Not tested in other applications.

Calculated MW

164 kDa. ( Note )

Positive Control

HeLa , A549

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Centrosomal Protein 164 , Nphp15 , Cep164

Cellular Localization

Localizes specifically to very distally located appendage structures on the mature centriole from which initiate PC formation. Persisted at centrioles throughout mitosis. Expressed in chromatin-enriched nuclear fraction of HeLa cells.

Background

This monoclonal antibody binds to Cep164 or centrosonal protein 164kDa. Cep164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA,CHK2 and CHK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHK1. [from UniProt]

Database

Research Area

DATA IMAGES

Anti-CEP164 antibody [17] used in Western Blot (WB). GTX70096

GTX70096 WB Image

Cep 164 antibody detects Cep 164 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Cep 164 antibody (GTX70096) diluted by 1:10000.

Anti-CEP164 antibody [17] used in Western Blot (WB). GTX70096

GTX70096 WB Image

Sample (30 ug of whole cell lysate)

A: HeLa

5% SDS PAGE

GTX70096 diluted at 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329