*Optimal dilutions/concentrations should be determined by the researcher.
Formaldehyde-fixed cell lines derived from Prostate Cancer and from Breast Cancer show typical centrosomal staining.
Not tested in other applications.
The peptide used to generate this antibody is available for purchase (GTX88258-PEP).
Despite similarity of the immunizing peptide with the mouse protein, this antibody was demonstrated to weak for mouse Cep290.
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide with sequence C-RNSKHLKQQQYRAENff, from the internal region of the protein sequence according to NP_079390.3.
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Centrosomal Protein 290,3H11Ag,Bbs14,Ct87,Jbts5,Lca10,Mks4,Nphp6,Poc3,Slsn6,Rd16,Cep290
Connecting cilium of photoreceptor cells, base of cilium in kidney intramedullary collecting duct cells.
This gene encodes a protein with 13 putative coiled-coil domains, a region with homology to SMC chromosome segregation ATPases, six KID motifs, three tropomyosin homology domains and an ATP/GTP binding site motif A. The protein is localized to the centrosome and cilia and has sites for N-glycosylation, tyrosine sulfation, phosphorylation, N-myristoylation, and amidation. Mutations in this gene have been associated with Joubert syndrome and nephronophthisis and the presence of antibodies against this protein is associated with several forms of cancer. [provided by RefSeq, Jul 2008]
GTX88258 ICC/IF Image
ICC/IF analysis of OPCT-1 (A) and MDA468 (B) cells using GTX88258 CEP290 antibody, Internal.
Dilution : 2.5µg/ml