APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 2-3μg/ml |
|
Note :
ICC/IF
Formaldehyde-fixed cell lines derived from Prostate Cancer and from Breast Cancer show typical centrosomal staining.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88258-PEP).
Product Note
Despite similarity of the immunizing peptide with the mouse protein, this antibody was demonstrated to weak for mouse Cep290.
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-RNSKHLKQQQYRAENff, from the internal region of the protein sequence according to NP_079390.3.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
centrosomal protein 290 , 3H11Ag , BBS14 , CT87 , JBTS5 , LCA10 , MKS4 , NPHP6 , POC3 , SLSN6 , rd16
Cellular Localization
Nucleus , Cytoplasmic vesicle
Background
This gene encodes a protein with 13 putative coiled-coil domains, a region with homology to SMC chromosome segregation ATPases, six KID motifs, three tropomyosin homology domains and an ATP/GTP binding site motif A. The protein is localized to the centrosome and cilia and has sites for N-glycosylation, tyrosine sulfation, phosphorylation, N-myristoylation, and amidation. Mutations in this gene have been associated with Joubert syndrome and nephronophthisis and the presence of antibodies against this protein is associated with several forms of cancer. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX88258 ICC/IF Image
ICC/IF analysis of OPCT-1 (A) and MDA468 (B) cells using GTX88258 CEP290 antibody, Internal.
Dilution : 2.5μg/ml
|
REFERENCE
There are currently no references for CEP290 antibody, Internal (GTX88258). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CEP290 antibody, Internal (GTX88258). Be the first to share your experience with this product.
