GeneTex
United States (US)

CFAB Bb (cleaved Lys260) antibody

CFAB-Bb-cleaved-Lys260-antibody-GTX86947-WB-1_18121409_677.jpg

Cat No. GTX86947

Host Rabbit
Clonality Polyclonal
Application WB, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

57 and 85 kDa. ( Note )

Specificity/Sensitivity

CFAB Bb (Cleaved-Lys260) Antibody detects endogenous levels of fragment of activated CFAB Bb resulting from cleavage adjacent to Lys260.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human CFAB Bb.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Complement Factor B,Ahus4,Armd14,Bf,Bfd,Cfab,Cfbd,Fb,Fbi12,Gbg,H2-Bf,Pbf2,Cfb

Background

Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.

Database

Research Area

DATA IMAGES
CFAB-Bb-cleaved-Lys260-antibody-GTX86947-WB-1_18121409_677.jpg

GTX86947 WB Image

Western blot analysis of extracts from K562 cells, treated with etoposide 25uM 1h, using CFAB Bb (Cleaved-Lys260) Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 349