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CFDP1 antibody

Anti-CFDP1 antibody used in Western Blot (WB). GTX128023
Anti-CFDP1 antibody used in Western Blot (WB). GTX128023
Anti-CFDP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128023
Anti-CFDP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX128023
Anti-CFDP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128023

Cat No. GTX128023

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375

Predict Reactivity

Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CFDP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

craniofacial development protein 1 , BCNT , BUCENTAUR , CENP-29 , CP27 , SWC5 , Yeti , p97

Background

May play a role during embryogenesis.

Database

Research Area

DATA IMAGES

Anti-CFDP1 antibody used in Western Blot (WB). GTX128023

GTX128023 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CFDP1 antibody (GTX128023) diluted at 1:3000.

Anti-CFDP1 antibody used in Western Blot (WB). GTX128023

GTX128023 WB Image

CFDP1 antibody detects CFDP1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
12 % SDS-PAGE
CFDP1 antibody (GTX128023) dilution: 1:1000

Anti-CFDP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX128023

GTX128023 IHC-P Image

CFDP1 antibody detects CFDP1 protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
CFDP1 antibody (GTX128023) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CFDP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX128023

GTX128023 IHC-Fr Image

CFDP1 antibody detects CFDP1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse mouse cerebellum.
Green: CFDP1 stained by CFDP1 antibody (GTX128023) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-CFDP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128023

GTX128023 ICC/IF Image

CFDP1 antibody detects CFDP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CFDP1 stained by CFDP1 antibody (GTX128023) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Scale bar= 10μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169