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CFHL1 antibody [N1C2]

Anti-CFHL1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118620
Anti-CFHL1 antibody [N1C2] used in Western Blot (WB). GTX118620

Cat. No. GTX118620

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CFHL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A549

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CFHL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10630076

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

complement factor H related 1 , CFHL , CFHL1 , CFHL1P , CFHR1P , FHR1 , H36-1 , H36-2 , HFL1 , HFL2

Cellular Localization

Secreted

Background

This gene encodes a secreted protein belonging to the complement factor H protein family. It binds to Pseudomonas aeruginosa elongation factor Tuf together with plasminogen, which is proteolytically activated. It is proposed that Tuf acts as a virulence factor by acquiring host proteins to the pathogen surface, controlling complement, and facilitating tissue invasion. Mutations in this gene are associated with an increased risk of atypical hemolytic-uremic syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CFHL1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118620

GTX118620 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CFHL1(GTX118620) antibody at 1:200 dilution.

Anti-CFHL1 antibody [N1C2] used in Western Blot (WB). GTX118620

GTX118620 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX118620 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169