APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.8 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide (C)KEETEEEVQDTRL, corresponding to amino acid residues 1468-1480 (Cytoplasmic, C-terminal part) of human CFTR (Accession P13569).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cystic fibrosis transmembrane conductance regulator , ABC35 , ABCC7 , CF , CFTR/MRP , MRP7 , TNR-CFTR , dJ760C5.1
Cellular Localization
Apical cell membrane,Cell membrane,Endoplasmic reticulum membrane,Nucleus
Background
This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
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GTX54774 IHC-P Image
IHC-P analysis of rat lung tissue using GTX54774 CFTR antibody. Strong staining of bronchial epithelial cells (red) and lighter staining of alveolar cells (red-brown) is apparent. There is also positive staining of macrophages while smooth muscle and endothelium are negative. Counterstain of cell nuclei appears blue. A negative control is shown in the right panel.
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GTX54774 WB Image
WB analysis of rat lung membrane lysate using GTX54774 CFTR antibody preincubated with or without immunogen peptide.
Dilution : 1:200
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REFERENCE
There are currently no references for CFTR antibody (GTX54774). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CFTR antibody (GTX54774). Be the first to share your experience with this product.
