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CFTR antibody

Anti-CFTR antibody used in Western Blot (WB). GTX87672
Anti-CFTR antibody used in IHC (Paraffin sections) (IHC-P). GTX87672
Anti-CFTR antibody used in Western Blot (WB). GTX87672
Anti-CFTR antibody used in Western Blot (WB). GTX87672

Cat. No. GTX87672

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all CFTR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF Assay dependent
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

168 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human CFTR (711-760).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

RRID

AB_10725899

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ABC35 , ABCC7 , CF , CFTR , CFTR/MRP , MRP7 , TNRCFTR , cystic fibrosis transmembrane conductance regulator , dJ760C5.1

Cellular Localization

Apical cell membrane,Early endosome membrane,Cell membrane,Recycling endosome membrane,Endoplasmic reticulum membrane

Background

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tissues. Channel activation is mediated by cycles of regulatory domain phosphorylation, ATP-binding by the nucleotide-binding domains, and ATP hydrolysis. Mutations in this gene cause cystic fibrosis, the most common lethal genetic disorder in populations of Northern European descent. The most frequently occurring mutation in cystic fibrosis, DeltaF508, results in impaired folding and trafficking of the encoded protein. Multiple pseudogenes have been identified in the human genome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-CFTR antibody used in Western Blot (WB). GTX87672

GTX87672 WB Image

WB analysis of SH-SY5Y cell lysates using GTX87672 CFTR antibody.
Dilution : 1:2000

Anti-CFTR antibody used in IHC (Paraffin sections) (IHC-P). GTX87672

GTX87672 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX87672 CFTR antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CFTR antibody used in Western Blot (WB). GTX87672

GTX87672 WB Image

WB analysis of 293T cell lysate using GTX87672 CFTR antibody.
Dilution : 1:2000

Anti-CFTR antibody used in Western Blot (WB). GTX87672

GTX87672 WB Image

WB analysis of NIH/3T3 cell lysates using GTX87672 CFTR antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

Application Reference

Kai Du et al. Journal of Cystic Fibrosis 2014; Aggregates of mutant CFTR fragments in airway epithelial cells of CF lungs: New pathologic observations
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399