GeneTex
United States (US)

CFTR antibody

CFTR-antibody-GTX87672-WB-1_18121409_943.jpg
CFTR-antibody-GTX87672-IHC-1_18121409_306.jpg

Cat No. GTX87672

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, IHC-P, ELISA, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:1000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

168 kDa. ( Note )

Specificity/Sensitivity

CFTR (Ab-737) Antibody detects endogenous levels of total CFTR protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human CFTR.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cystic Fibrosis Transmembrane Conductance Regulator,Abc35,Abcc7,Cf,Cftr/Mrp,Mrp7,Tnr-Cftr,Dj760C5.1,Cftr

Background

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene.

Database

Research Area

DATA IMAGES
CFTR-antibody-GTX87672-WB-1_18121409_943.jpg

GTX87672 WB Image

Western blot analysis of extracts from NIH-3T3 cells, using CFTR (Ab-737) Antibody. The lane on the right is treated with the synthesized peptide.

CFTR-antibody-GTX87672-IHC-1_18121409_306.jpg

GTX87672 IHC-P Image

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using CFTR (Ab-737) Antibody. The picture on the right is treated with the synthesized peptide.

REFERENCE
Package List Price ($)
$ 339