Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with CG1458 antibody (GTX122151) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)

A: Drosophila melanogaster, adult (wild type)

B: Cisd2 knock down (actin promoter)
C: Cisd2 over express (actin promoter)

15% SDS PAGE

GTX122151 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CG1458 antibody(GTX122151) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min