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CGRP antibody

Anti-CGRP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80742

Cat No. GTX80742

Host Chicken
Clonality Polyclonal
Application ICC/IF, IHC
Reactivity Rat
Package
50 μl ($319)
APPLICATION

Application Note

Immunohistochemistry: 1:100 -1:500 Dilutions listed as a recommendation. Optimal dilution should be determined by investigator. Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free TyrodeÕs solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4oC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy.

Calculated MW

15 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Whole Serum

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Calcitonin Related Polypeptide Alpha,Calc1,Cgrp,Cgrp-I,Cgrp1,Ct,Kc,Pct,Calca

Background

Isolated Chicken IgY
DATA IMAGES
Anti-CGRP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80742

GTX80742 ICC/IF Image

Expression of calcitonin gene-related peptide (CGRP) and SNAP-25 in trigeminal ganglion neurons. The expression of CGRP (A) and SNAP-25 (B) are shown in the entire trigeminal ganglion obtained from an untreated adult animal. At higher magnification, neuronal processes(arrows) expressing both CGRP (C) and SNAP-25 (D)that extend from neuronal cell bodies in one cluster to another cluster are observed. Neuronal processes expressing SNAP-25 are also observed within neuronal clusters (E)

REFERENCE
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REVIEW
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Package List Price ($)
$ 319