APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.2-2.5 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
293T , HepG2 , human fetal liver , human placenta , mouse brain
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a N-terminal region of Human CHAF1B
Purification
Affinity Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
chromatin assembly factor 1 subunit B , CAF-1 , CAF-IP60 , CAF1 , CAF1A , CAF1P60 , MPHOSPH7 , MPP7
Cellular Localization
Nucleus,Cytoplasm
Background
Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX44690 WB Image
WB analysis of human placenta tissue using GTX44690 CHAF1B antibody at 1.0μg/ml.
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GTX44690 WB Image
WB analysis of mouse brain tissue using GTX44690 CHAF1B antibody at 1:1000.
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GTX44690 WB Image
WB analysis of 293T cells using GTX44690 CHAF1B antibody at 1.0μg/ml.
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GTX44690 WB Image
WB analysis of human fetal liver tissue using GTX44690 CHAF1B antibody at 0.2-1μg/ml.
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GTX44690 WB Image
WB analysis of HepG2 cells using GTX44690 CHAF1B antibody at 1.0μg/ml.
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REFERENCE
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REVIEW
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