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CHAF1B antibody

Anti-CHAF1B antibody used in IHC (Paraffin sections) (IHC-P). GTX65808
Anti-CHAF1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65808
Anti-CHAF1B antibody used in Western Blot (WB). GTX65808
Anti-CHAF1B antibody used in IHC (Paraffin sections) (IHC-P). GTX65808
Anti-CHAF1B antibody used in IHC (Paraffin sections) (IHC-P). GTX65808

Cat. No. GTX65808

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CHAF1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 270-559 of human CHAF1B (NP_005432.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromatin assembly factor 1 subunit B , CAF-1 , CAF-IP60 , CAF1 , CAF1A , CAF1P60 , MPHOSPH7 , MPP7

Cellular Localization

Nucleus,Cytoplasm

Background

Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CHAF1B antibody used in IHC (Paraffin sections) (IHC-P). GTX65808

GTX65808 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX65808 CHAF1B antibody.
Dilution : 1:100

Anti-CHAF1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65808

GTX65808 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX65808 CHAF1B antibody.
Blue : DAPI

Anti-CHAF1B antibody used in Western Blot (WB). GTX65808

GTX65808 WB Image

WB analysis of various sample lysates using GTX65808 CHAF1B antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CHAF1B antibody used in IHC (Paraffin sections) (IHC-P). GTX65808

GTX65808 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX65808 CHAF1B antibody.
Dilution : 1:100

Anti-CHAF1B antibody used in IHC (Paraffin sections) (IHC-P). GTX65808

GTX65808 IHC-P Image

IHC-P analysis of rat fallopian tube tissue using GTX65808 CHAF1B antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399