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CHCHD3 antibody

Anti-CHCHD3 antibody used in Immunoprecipitation (IP). GTX64550
Anti-CHCHD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64550
Anti-CHCHD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64550
Anti-CHCHD3 antibody used in Western Blot (WB). GTX64550
Anti-CHCHD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64550
Anti-CHCHD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64550

Cat. No. GTX64550

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:100
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human CHCHD3 (NP_060282.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coiled-coil-helix-coiled-coil-helix domain containing 3 , MICOS19 , MINOS3 , Mic19 , PPP1R22

Cellular Localization

Mitochondrion inner membrane,Cytoplasm,Nucleus,Mitochondrion

Background

The protein encoded by this gene is an inner mitochondrial membrane scaffold protein. Absence of the encoded protein affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. This protein is part of the mitochondrial contact site and cristae organizing system (MICOS). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-CHCHD3 antibody used in Immunoprecipitation (IP). GTX64550

GTX64550 IP Image

IP analysis of mouse liver tissue lysate using GTX64550 CHCHD3 antibody.
Antibody amount : 3μg / 600μg lysate
Dilution : 1:1000

Anti-CHCHD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64550

GTX64550 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX64550 CHCHD3 antibody.
Dilution : 1:100

Anti-CHCHD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64550

GTX64550 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX64550 CHCHD3 antibody.
Dilution : 1:100

Anti-CHCHD3 antibody used in Western Blot (WB). GTX64550

GTX64550 WB Image

WB analysis of various sample lysates using GTX64550 CHCHD3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CHCHD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX64550

GTX64550 IHC-P Image

IHC-P analysis of rat brain tissue using GTX64550 CHCHD3 antibody.
Dilution : 1:100

Anti-CHCHD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64550

GTX64550 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64550 CHCHD3 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399