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CHD1L antibody [N2C1], Internal

Anti-CHD1L antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX119360
Anti-CHD1L antibody [N2C1], Internal used in Western Blot (WB). GTX119360
Anti-CHD1L antibody [N2C1], Internal used in Western Blot (WB). GTX119360

Cat No. GTX119360

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

293T , HepG2 , HepG2 nucleus

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CHD1L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

chromodomain helicase DNA binding protein 1 like,ALC1,CHDL

Cellular Localization

Nucleus

Background

In response to DNA strand breaks, chromatin adopts a relaxed structure due to the addition of poly(ADP-ribose) (PAR) to chromatin proteins by PARP enzymes (see PARP1; MIM 173870), and this relaxation facilitates the repair of DNA damage. CHD1L interacts with PAR and has a role in chromatin relaxation following DNA damage (Ahel et al., 2009 [PubMed 19661379]).[supplied by OMIM]

Database

DATA IMAGES
Anti-CHD1L antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX119360

GTX119360 IP Image

CHD1L antibody immunoprecipitates CHD1L protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of CHD1L protein by 2 μg of CHD1L antibody (GTX119360) 7.5% SDS-PAGE The immunoprecipitated CHD1L protein was detected by CHD1L antibody (GTX119360) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CHD1L antibody [N2C1], Internal used in Western Blot (WB). GTX119360

GTX119360 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
B: HepG2 nucleus
5% SDS PAGE
GTX119360 diluted at 1:3000

Anti-CHD1L antibody [N2C1], Internal used in Western Blot (WB). GTX119360

GTX119360 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CHD1L antibody [N2C1], Internal (GTX119360) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169