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CHD1L antibody

Anti-CHD1L antibody used in Western Blot (WB). GTX129527
Anti-CHD1L antibody used in Western Blot (WB). GTX129527

Cat No. GTX129527

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CHD1L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

chromodomain helicase DNA binding protein 1 like , ALC1 , CHDL

Cellular Localization

Nucleus

Background

In response to DNA strand breaks, chromatin adopts a relaxed structure due to the addition of poly(ADP-ribose) (PAR) to chromatin proteins by PARP enzymes (see PARP1; MIM 173870), and this relaxation facilitates the repair of DNA damage. CHD1L interacts with PAR and has a role in chromatin relaxation following DNA damage (Ahel et al., 2009 [PubMed 19661379]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-CHD1L antibody used in Western Blot (WB). GTX129527

GTX129527 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CHD1L antibody (GTX129527) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CHD1L antibody used in Western Blot (WB). GTX129527

GTX129527 WB Image

CHD1L antibody detects CHD1L protein by western blot analysis.
A. 30 μg 293T whole cell extract |
B. 30 μg A431 whole cell extract
C. 30 μg HeLa whole cell extract
D. 30 μg HepG2 whole cell extract
7.5 % SDS-PAGE
CHD1L antibody (GTX129527) dilution: 1:3000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf