CHD3 antibody [2G4]

Cat. No. GTX83232

Host	Mouse
Clonality	Monoclonal
Clone Name	2G4
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse
Package	100 μg ($399)

See all CHD3 products

Datasheet
Document

View product citations for antibody GTX83232 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

227 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CHD3 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromodomain helicase DNA binding protein 3 , Mi-2a , Mi2-ALPHA , SNIBCPS , ZFH

Cellular Localization

Nucleus

Background

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Gene ID: 1107 CHD3
UniProt: Q12873 CHD3

Research Area

DATA IMAGES

GTX83232 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX83232 CHD3 antibody [2G4].
Green : CHD3
Blue: DRAQ5 fluorescent DNA dye

GTX83232 FACS Image

FACS analysis of K562 cells using GTX83232 CHD3 antibody [2G4].
Green : CHD3
Purple : negative control

GTX83232 IHC-P Image

IHC-P analysis of colon cancer tissue using GTX83232 CHD3 antibody [2G4].

GTX83232 WB Image

WB analysis of HeLa (1), K562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate using GTX83232 CHD3 antibody [2G4].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty