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CHD3 antibody

Anti-CHD3 antibody used in Western Blot (WB). GTX131779
Anti-CHD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131779
Anti-CHD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131779

Cat No. GTX131779

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

227 kDa. ( Note )

Positive Control

293T , A431 , HeLa
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CHD3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

chromodomain helicase DNA binding protein 3,Mi-2a,Mi2-ALPHA,SNIBCPS,ZFH

Cellular Localization

Nucleus

Background

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CHD3 antibody used in Western Blot (WB). GTX131779

GTX131779 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD3 antibody (GTX131779) diluted at 1:1000.

Anti-CHD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX131779

GTX131779 IHC-P Image

CHD3 antibody detects CHD3 protein at cytoplasm and nucleus in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CHD3 antibody (GTX131779) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CHD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131779

GTX131779 ICC/IF Image

CHD3 antibody detects CHD3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CHD3 protein stained by CHD3 antibody (GTX131779) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169