Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD4 antibody [HL1798] (GTX637450) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CHD4 antibody [HL1798] detects CHD4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ductus deferens.
CHD4 stained by CHD4 antibody [HL1798] (GTX637450) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD4 antibody [HL1798] (GTX637450) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.