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CHD4 antibody [HL1798]

Recombinant antibody Orthogonal
Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450
Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450
Anti-CHD4 antibody [HL1798] used in IHC (Paraffin sections) (IHC-P). GTX637450

Cat. No. GTX637450

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1798

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CHD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

218 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of Human CHD4.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chromodomain helicase DNA binding protein 4 , CHD-4 , Mi-2b , Mi2-BETA , SIHIWES

Cellular Localization

Nucleus,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Background

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

Database

Research Area

DATA IMAGES

Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450

GTX637450 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD4 antibody [HL1798] (GTX637450) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CHD4 antibody [HL1798] used in Western Blot (WB). GTX637450

GTX637450 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CHD4 antibody [HL1798] (GTX637450) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CHD4 antibody [HL1798] used in IHC (Paraffin sections) (IHC-P). GTX637450

GTX637450 IHC-P Image

CHD4 antibody [HL1798] detects CHD4 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ductus deferens.
CHD4 stained by CHD4 antibody [HL1798] (GTX637450) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations